Protocol: Targeted isolation of circular extrachromosomal DNA by CRISPR-CATCH
This protocol enables targeted isolation of circular extrachromosomal DNA using CRISPR-Cas9-mediated linearization followed by pulsed field gel electrophoresis. Isolated DNA products can be subsequently used for short-read sequencing, single-molecule long-read sequencing or other assays.
Original research article: Hung KL et al. Nature Genetics. 2022
Tool: Using AmpliconArchitect for CRISPR-CATCH
This tool was developed for reconstruction of circular extrachromosomal amplicon structures using short-read sequencing data from CRISPR-CATCH.
Original research article: Hung KL et al. Nature Genetics. 2022